Author: Kenneth Lyon; Luis U. Aguilera; Tatsuya Morisaki; Brian Munsky; Timothy J. Stasevich
Title: Live-cell single RNA imaging reveals bursts of translational frameshifting Document date: 2018_11_24
ID: 4fm1skgh_102
Snippet: To track single molecule translation sites, a custom-built widefield fluorescence microscope based on a highly inclined illumination scheme (Tokunaga et al., 2008 ) was used (Morisaki et al., 2016 . Briefly, the excitation beams, 488, 561 and 637 nm solidstate lasers (Vortran), were coupled and focused at the back focal plane of the objective lens (60X, NA 1.49 oil immersion objective, Olympus). The emission signals were split by an imaging grade.....
Document: To track single molecule translation sites, a custom-built widefield fluorescence microscope based on a highly inclined illumination scheme (Tokunaga et al., 2008 ) was used (Morisaki et al., 2016 . Briefly, the excitation beams, 488, 561 and 637 nm solidstate lasers (Vortran), were coupled and focused at the back focal plane of the objective lens (60X, NA 1.49 oil immersion objective, Olympus). The emission signals were split by an imaging grade, ultra-flat dichroic mirror (T660lpxr, Chroma) and detected using two All rights reserved. No reuse allowed without permission.
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