Author: Václav Vopálenský; Michal Sýkora; Tomáš Mašek; Martin Pospíšek
Title: Messenger RNAs transcribed from yeast linear cytoplasmic plasmids possess unconventional 5’ and 3’ UTRs and suggest a novel mechanism of translation Document date: 2018_5_17
ID: foskvkwn_112_1
Snippet: thesis present on both sides of the plasmid sequence indicate the position of the DNA sequence relative to the GenBank records indicated above. Unique obtained sequences (prepared using SuperScript III Reverse Transcriptase) corresponding to transcripts of a given ORF are aligned under the template DNA. The sequences of at least 20 independent clones were determined and aligned with the plasmid (template) DNA of each ORF......
Document: thesis present on both sides of the plasmid sequence indicate the position of the DNA sequence relative to the GenBank records indicated above. Unique obtained sequences (prepared using SuperScript III Reverse Transcriptase) corresponding to transcripts of a given ORF are aligned under the template DNA. The sequences of at least 20 independent clones were determined and aligned with the plasmid (template) DNA of each ORF.
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