Real-time reverse transcription loop-mediated isothermal amplification for rapid detection of SARS-CoV-2

Lau, Yee Ling; Ismail, Ilyiana; Mustapa, Nur Izati; Lai, Meng Yee; Tuan Soh, Tuan Suhaila; Hassan, Afifah; Peariasamy, Kalaiarasu M.; Lee, Yee Leng; Chong, Yoong Min; Sam, I-Ching; Goh, Pik Pin

BACKGROUND: Highly sensitive real-time reverse transcription polymerase chain reaction (RT-qPCR) methods have been developed for the detection of SARS-CoV-2. However, they are costly. Loop-mediated isothermal amplification (LAMP) assay has emerged as a novel alternative isothermal amplification method for the detection of nucleic acid. METHODS: A rapid, sensitive and specific real-time reverse transcription LAMP (RT-LAMP) assay was developed for SARS-CoV-2 detection. RESULTS: This assay detected one copy/reaction of SARS-CoV-2 RNA in 30 min. Both the clinical sensitivity and specificity of this assay were 100%. The RT-LAMP showed comparable performance with RT-qPCR. Combining simplicity and cost-effectiveness, this assay is therefore recommended for use in resource resource-limited settings.