Author: Chad N. Brocker; Donghwan Kim; Tisha Melia; Kritika Karri; Thomas J. Velenosi; Shogo Takahashi; Jessica A. Bonzo; David J. Waxman; Frank J. Gonzalez
Title: Long non-coding RNA Gm15441 attenuates hepatic inflammasome activation in response to metabolic stress Document date: 2019_6_20
ID: dt0b7jnu_63
Snippet: Total RNA was isolated from fresh mouse liver, mouse primary hepatocytes, and Hepa-1 cells using TRIzol Reagent (Thermo-Fisher Scientific, Waltham, MA, USA) and quantified using a NanoDrop Spectrophotometer (NanoDrop Products, Wilmington, DE, USA). Total RNA (2 µg) was reverse transcribed using All-in-One cDNA Synthesis SuperMix (BioTool, Houston, TX, . CC-BY-NC-ND 4.0 International license is made available under a The copyright holder for this.....
Document: Total RNA was isolated from fresh mouse liver, mouse primary hepatocytes, and Hepa-1 cells using TRIzol Reagent (Thermo-Fisher Scientific, Waltham, MA, USA) and quantified using a NanoDrop Spectrophotometer (NanoDrop Products, Wilmington, DE, USA). Total RNA (2 µg) was reverse transcribed using All-in-One cDNA Synthesis SuperMix (BioTool, Houston, TX, . CC-BY-NC-ND 4.0 International license is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It . https://doi.org/10.1101/675785 doi: bioRxiv preprint USA). qRT-PCR analysis was performed using SYBR Green qPCR Master Mix (BioTool).
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