Selected article for: "Ct value and qrt pcr"
Author: Chad N. Brocker; Donghwan Kim; Tisha Melia; Kritika Karri; Thomas J. Velenosi; Shogo Takahashi; Jessica A. Bonzo; David J. Waxman; Frank J. Gonzalez
Title: Long non-coding RNA Gm15441 attenuates hepatic inflammasome activation in response to metabolic stress
  • Document date: 2019_6_20
    • ID: dt0b7jnu_64
    Snippet: Primers were designed for gene specificity and to cross exon-exon junctions using Primer-BLAST (www.ncbi.nlm.nih.gov/tools/primer-blast/) and purchased from IDT DNA Technologies (Coralville, IA, USA) ( Table S4) . qRT-PCR experiments were designed and performed according to Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines (Plain et al., 2014) . Results are normalized to actin expression. Values given.....
    Document: Primers were designed for gene specificity and to cross exon-exon junctions using Primer-BLAST (www.ncbi.nlm.nih.gov/tools/primer-blast/) and purchased from IDT DNA Technologies (Coralville, IA, USA) ( Table S4) . qRT-PCR experiments were designed and performed according to Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines (Plain et al., 2014) . Results are normalized to actin expression. Values given are fold over control or relative expression value, where appropriate, calculated using the 2ΔCt QPCR calculation method (Pfaffl, 2001) .

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