Long non-coding RNA Gm15441 attenuates hepatic inflammasome activation in response to metabolic stress

Chad N. Brocker; Donghwan Kim; Tisha Melia; Kritika Karri; Thomas J. Velenosi; Shogo Takahashi; Jessica A. Bonzo; David J. Waxman; Frank J. Gonzalez

Analysis of the mapped sequence reads obtained by stranded RNA sequencing revealed a pronounced increase in expression of Gm15441 (Figure 2A) , whereas, the Txnip transcript on the opposite strand was suppressed by WY-14643 at the time point analyzed ( Figure 2B) . qRT-PCR analysis confirmed the very large, highly significant increase in Gm15441 expression and its complete inhibition in Ppara -/liver ( Figure 2C) . Further, Txnip RNA was significantly suppressed by WY-14643 in wild-type mice ( Figure 2D) . Additionally, Txnip expression was attenuated in untreated Ppara -/compared to Ppara +/+ mouse liver, indicating a role for PPARA in maintaining basal expression of Txnip ( Figure 2D ). Given that Txnip expression can be upregulated in human neuroblastoma cells by the PPARA activator fenofibrate, which suppresses proliferation and migration (Su et al., 2015) , the effects of a single dose of WY-14643 on Gm15441