Long noncoding RNA AVAN promotes antiviral innate immunity by interacting with TRIM25 and enhancing the transcription of FOXO3a

Chengcai Lai; Lihui Liu; Qinghua Liu; Sijie Cheng; Keyu Wang; Lingna Zhao; Min Xia; Cheng Wang; Hongjing Gu; Yueqiang Duan; Zhongpeng Zhao; Lili Zhang; Ziyang Liu; Jianjun Luo; Jianxun Song; Penghui Yang; Runsheng Chen; Xiliang Wang

The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. . https://doi.org/10.1101/623132 doi: bioRxiv preprint 548 RIP assays were performed as described (51) with minor modification. Briefly, after 549 incubation, the magnetic beads were washed with high salt lysis buffer (containing 500mM 550 NaCl) 5 times. RIP products were analyzed by qRT-PCR using the primer pairs listed in Table 551 S12. ChIP was performed as described (67). The ChIP-enriched FOXO3a promoter was 552 quantified by qPCR using the primer pairs listed in Table S12 . LacZ as control. Next, C-1 magnetic beads were added to the probe-chromatin mixture. After 565 five total washes with washing buffer (2×SSC, 0.5% SDS, add DTT and PMSF before use), 566 the beads were separated into two parts, 1/10 for RNA elution and 9/10 for DNA elution or 567 protein elution. For elution of the RNA, the beads were treated with Protease K Buffer and 568 All rights reserved. No reuse allowed without permission.