Author: Václav Vopálenský; Michal Sýkora; Tomáš Mašek; Martin Pospíšek
Title: Messenger RNAs transcribed from yeast linear cytoplasmic plasmids possess unconventional 5’ and 3’ UTRs and suggest a novel mechanism of translation Document date: 2018_5_17
ID: foskvkwn_89
Snippet: Production of the pGKL1 killer toxin from K. lactis IFO1267, IFO1267 pbp1Δ, IFO1267 lsm1Δ and IFO1267 pbp1Δ pab1Δ strains to culture medium in early stationary phase (~51 hours), when all the cultures reached comparable OD 600 (see Fig. 7 ). Production of the pGKL1 killer toxin into culture medium was assayed by a well diffusion test on YPD agar plates with a lawn of the S. cerevisiae S6/1 sensitive strain. Culture medium was used either undi.....
Document: Production of the pGKL1 killer toxin from K. lactis IFO1267, IFO1267 pbp1Δ, IFO1267 lsm1Δ and IFO1267 pbp1Δ pab1Δ strains to culture medium in early stationary phase (~51 hours), when all the cultures reached comparable OD 600 (see Fig. 7 ). Production of the pGKL1 killer toxin into culture medium was assayed by a well diffusion test on YPD agar plates with a lawn of the S. cerevisiae S6/1 sensitive strain. Culture medium was used either undiluted (1x) or diluted 10x and 100x with sterile YPD medium. This figure refers to Fig. 7 .
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