Determination of the Near-Atomic Structure of Non-Purified Oligomeric E. coli Proteins

Pichkur, E. B.; Mikirtumov, V. I.; Tikhonova, O. V.; Derkacheva, N. I.; Kurochkina, L. P.; Sokolova, O. S.

The roughly purified extract of E. coli proteins has been studied by cryoelectron microscopy, the class-sums containing 2D projections of two proteins (β-galactosidase and 2-oxoglutarate dehydrogenase complex catalytic domain (ODC-CD)), identified in an extract by tandem mass spectrometry, have been distinguished. The structures of these proteins have been solved at near-atomic resolution. De novo simulation of the ODC-CD structure yielded an atomic model that revealed differences in the positions of some amino acid residues of the active center, in comparison with the known crystal structures.