Microbial and autoantibody immunogenic repertoires in TIF1? autoantibody positive dermatomyositis

Spyridon Megremis; Thomas Walker; Xiaotong He; James O'Sullivan; William E.R. Ollier; Hector Chinoy; Neil Pendleton; Antony Payton; Lynne Hampson; Ian Hampson; Janine Lamb

The increase in the number of plasma samples within the pool used for cross-panning, from a pool of 10 (P10) to a pool of 20 (P20), had a differential effect between the two groups: in DM, an increase in the number of microbial species was observed from 728 (DM P10) to 832 (DM P20), whereas in the HC group a decrease was observed from 780 (HC P10) to 718 (HC P20) species. The number of epitopes per microbial species in both the DM and the HC significantly increased with increasing pooling size ( Figure 2A ). The number of NGS reads per microbial species significantly increased in the DM P20 (95% CI: 3.97-4.13) compared to DM P10 (95% CI: 3.90-3.98), whereas it did not change in the HC P20 (95% CI: 4.09-4.26) compared to the P10 (95% CI: 4.34-4.43) ( Figure 2B ).