Mammalian orthoreovirus infection is enhanced in cells pre-treated with sodium arsenite

Michael M. Lutz; Megan P. Worth; Meleana M. Hinchman; John S. L. Parker; Emily D. Ledgerwood

We found that stress granule formation in reovirus-infected CV-1 cells peaked around 6 h 240 p.i. with 6.6% of cells containing stress granules. By 8 h p.i. the percentage of infected 241 cells containing stress granules had dropped to 3.1% and by 18 h p.i., the level was no 242 different from mock-infected cells ( Figures 1A-B) . A previous report showed that stress 243 granules were present in reovirus-infected human prostate carcinoma DU145 cells at 19.5 244 h post infection [15] . Although we did not see stress granules in CV-1 cells at late times 245 post-infection with reovirus at an MOI = 1, we found that G3BP and TIAR co-localized 246 at the periphery of viral factories at 24 h p.i. when cells were treated with 0.5 mM sodium 247 arsenite for 30 min immediately prior to harvest ( Figure 1C) . We occasionally saw a 248 similar phenotype at 24 h p.i. in infected cells not treated with sodium arsenite ( we observed only a modest increase in the expression level of stress granule protein 253 All rights reserved. No reuse allowed without permission.