Author: Václav Vopálenský; Michal Sýkora; Tomáš Mašek; Martin Pospíšek
Title: Messenger RNAs transcribed from yeast linear cytoplasmic plasmids possess unconventional 5’ and 3’ UTRs and suggest a novel mechanism of translation Document date: 2018_5_17
ID: foskvkwn_11
Snippet: The pGEX4T2::CDC33 plasmid was transformed into the expression strain E. coli BL21(DE3) (Merck). A 500 ml culture was grown at 28°C in 2xTY medium containing ampicillin (100 μg/ml) until the OD 600 reached 0.20. At this point, the culture was cooled to 15°C, cultivated for 4 hours and then induced by 0.1 mM isopropyl β-Dthiogalactopyranoside. Production of recombinant protein was carried out with shaking at 15°C for another 14-16 hours. Lysa.....
Document: The pGEX4T2::CDC33 plasmid was transformed into the expression strain E. coli BL21(DE3) (Merck). A 500 ml culture was grown at 28°C in 2xTY medium containing ampicillin (100 μg/ml) until the OD 600 reached 0.20. At this point, the culture was cooled to 15°C, cultivated for 4 hours and then induced by 0.1 mM isopropyl β-Dthiogalactopyranoside. Production of recombinant protein was carried out with shaking at 15°C for another 14-16 hours. Lysates were prepared using B-PER Protein Extraction Reagents (Thermo Scientific) according to the manufacturer's instruction and stored at −70°C for further use.
Search related documents:
Co phrase search for related documents- coli strain and recombinant protein production: 1
- expression transform and recombinant protein: 1
Co phrase search for related documents, hyperlinks ordered by date