Author: Václav Vopálenský; Michal Sýkora; Tomáš Mašek; Martin Pospíšek
Title: Messenger RNAs transcribed from yeast linear cytoplasmic plasmids possess unconventional 5’ and 3’ UTRs and suggest a novel mechanism of translation Document date: 2018_5_17
ID: foskvkwn_117
Snippet: A reaction mixture (20 µl) containing 50 mM Tris-HCl (pH 8.0); 2 mM DTT; 20 mM MgCl 2 ; [α-32 P]GTP and purified K2Oof3p was incubated at 30°C for 60 min. The reaction was quenched with SDS-PAGE loading buffer, and the products were resolved by SDS-PAGE. The K2Orf3p-[ 32 P]GMP adduct was visualized by autoradiography of the dried gel......
Document: A reaction mixture (20 µl) containing 50 mM Tris-HCl (pH 8.0); 2 mM DTT; 20 mM MgCl 2 ; [α-32 P]GTP and purified K2Oof3p was incubated at 30°C for 60 min. The reaction was quenched with SDS-PAGE loading buffer, and the products were resolved by SDS-PAGE. The K2Orf3p-[ 32 P]GMP adduct was visualized by autoradiography of the dried gel.
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