Author: Václav Vopálenský; Michal Sýkora; Tomáš Mašek; Martin Pospíšek
Title: Messenger RNAs transcribed from yeast linear cytoplasmic plasmids possess unconventional 5’ and 3’ UTRs and suggest a novel mechanism of translation Document date: 2018_5_17
ID: foskvkwn_119
Snippet: Isogenic S. cerevisiae strains bearing the wild-type CDC33 gene (CWO4CDC33wt) and its temperature-sensitive mutations (CWO4cdc33-1 and CWO4cdc33-42, for exact genotypes see Table S3 ) were cultivated for two days in synthetic drop-out minimal medium (SD) that lacked leucine and tryptophan (SD-TL) and contained ethidium bromide at a final concentration of 25 µg per ml. Afterwards, these cultures were diluted five thousand times, cultivated again.....
Document: Isogenic S. cerevisiae strains bearing the wild-type CDC33 gene (CWO4CDC33wt) and its temperature-sensitive mutations (CWO4cdc33-1 and CWO4cdc33-42, for exact genotypes see Table S3 ) were cultivated for two days in synthetic drop-out minimal medium (SD) that lacked leucine and tryptophan (SD-TL) and contained ethidium bromide at a final concentration of 25 µg per ml. Afterwards, these cultures were diluted five thousand times, cultivated again for two days and then diluted and cultivated once more. Loss of mitochondria was verified by the lack of colony growth on medium with glycerol as the sole carbon source and by DAPI staining followed by fluorescence microscopy.
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