Author: Václav Vopálenský; Michal Sýkora; Tomáš Mašek; Martin Pospíšek
Title: Messenger RNAs transcribed from yeast linear cytoplasmic plasmids possess unconventional 5’ and 3’ UTRs and suggest a novel mechanism of translation Document date: 2018_5_17
ID: foskvkwn_23
Snippet: The first primary PCR product was amplified from a native pGKL2 plasmid using Pfu DNA polymerase (Fermentas) and the primers ORF3_SAM_del_F1 and ORF3_SAMdel_R1. The second primary PCR product was amplified using Pfu DNA polymerase (Fermentas) and the primers KL_orf6C_Flag2F and ORF3_SAMdel_R2 (see Table S2 ) from a modified pGKL1 plasmid whose K1ORF2 coding sequence had been replaced with G418 (unpublished data)......
Document: The first primary PCR product was amplified from a native pGKL2 plasmid using Pfu DNA polymerase (Fermentas) and the primers ORF3_SAM_del_F1 and ORF3_SAMdel_R1. The second primary PCR product was amplified using Pfu DNA polymerase (Fermentas) and the primers KL_orf6C_Flag2F and ORF3_SAMdel_R2 (see Table S2 ) from a modified pGKL1 plasmid whose K1ORF2 coding sequence had been replaced with G418 (unpublished data).
Search related documents:
Co phrase search for related documents- dna polymerase and PCR product: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15
- dna polymerase and Pfu dna polymerase: 1, 2, 3, 4, 5, 6
- Fermentas Pfu dna polymerase and PCR product: 1
- Fermentas Pfu dna polymerase and Pfu dna polymerase: 1, 2
- PCR product and Pfu dna polymerase: 1, 2
Co phrase search for related documents, hyperlinks ordered by date