Messenger RNAs transcribed from yeast linear cytoplasmic plasmids possess unconventional 5’ and 3’ UTRs and suggest a novel mechanism of translation

Václav Vopálenský; Michal Sýkora; Tomáš Mašek; Martin Pospíšek

(C) Tabular summary of 5' RACE experiments. A graphical representation of each experiment is in Table S1A and B. Each ORF is characterized by the following data: 1/ ORF number; 2/ number of 5' non-template adenosine nucleotides found in the corresponding mRNAs; 3/ minimal number of adenosine nucleotides (calculated for both template and non-template adenosines) found at 5' ends of uncapped transcripts; 4/ percentage of all transcripts containing 5' non-template adenosine nucleotides; 5/ the average number (shown as the mean and median) of non-template adenosine nucleotides per mRNA molecule; 6/ number of sequenced clones used for analyses; the number of capped and 5' polyadenylated mRNAs are depicted below the total number; 7/ percentage of all transcripts containing 5' mRNA cap structure; 8/ part of the 5' UTR complementary to the relevant plasmid genome. Please note that columns 4 and 7 may not sum to one hundred percent because some transcripts contain both 5' non-template adenosines and a cap structure. 1 template and non-template; 2 G = capped transcripts; A -5' polyadenylated transcripts; 3 first templated nucleotides transcribed to the mRNA, non-template adenosines were removed; * these adenosines immediately precede the ATG sequence; ** for exact sequences, see Table S1A ; transcripts from K. lactis ACT gene, coding for actin, were used as an internal control.