Selected article for: "efficient DICER cleavage and non templated addition"

Author: Ian M. Silverman; Sager J. Gosai; Nicholas Vrettos; Shawn W. Foley; Nathan D. Berkowitz; Zissimos Mourelatos; Brian D. Gregory
Title: Isolation and sequencing of AGO-bound RNAs reveals characteristics of mammalian stem-loop processing in vivo
  • Document date: 2018_4_6
  • ID: 1pbshnw9_3
    Snippet: The miRNA biogenesis mechanism is further complicated by the fact that both pre-and mature miRNAs can be post-transcriptionally modified at the 3' end by trimming or by non-templated addition of ribonucleotides, especially uridine and adenine (17) (18) (19) (20) (21) (22) (23) (24) . In general, mono-uridylation is thought to re-establish a 3' 2 nt overhang, which is required for efficient DICER cleavage. In contrast oligouridylation has been sho.....
    Document: The miRNA biogenesis mechanism is further complicated by the fact that both pre-and mature miRNAs can be post-transcriptionally modified at the 3' end by trimming or by non-templated addition of ribonucleotides, especially uridine and adenine (17) (18) (19) (20) (21) (22) (23) (24) . In general, mono-uridylation is thought to re-establish a 3' 2 nt overhang, which is required for efficient DICER cleavage. In contrast oligouridylation has been shown to be a signal for degradation and usually occurs after AGO2-mediated slicing of pre-miRNAs. However, most detailed studies of pre-miRNAs have been performed on a small number of pre-miRNA sequences, thus our understanding of the overall landscape, and global function of these modifications remains elusive.

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