Loss of IKK subunits limits NF-?B signaling in reovirus infected cells

Andrew J. McNamara; Pranav Danthi

While our work did not directly test these ideas, our gene expression analyses indicate 291 that reovirus does not inhibit the function of IRF3 (Fig. 1) . We also do not observe 292 . CC-BY 4.0 International license is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It . https://doi.org/10.1101/843680 doi: bioRxiv preprint inhibition of the transcriptional complex that contains IRF9 (not shown). Because these 293 previous studies were performed in different cell types and used different reovirus 294 strains, we propose that reovirus has evolved multiple mechanisms to dampen the 295 innate immune response. Our study presented here unveils one such mechanism. 296 . CC-BY 4.0 International license is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It were observed in permissivity between ATCC L929 cells and spinner-adapted L929 308 cells. A laboratory stock of T3A (obtained from T. Dermody's laboratory) was used for 309 infections. Infectious viral particles were purified by Vertrel XF extraction and CsCl 310 gradient centrifugation (41). Viral titer was determined by a plaque assay using spinner-311 adapted L929 cell with chymotrypsin in the agar overlay. 312