Microbial and autoantibody immunogenic repertoires in TIF1? autoantibody positive dermatomyositis

Spyridon Megremis; Thomas Walker; Xiaotong He; James O'Sullivan; William E.R. Ollier; Hector Chinoy; Neil Pendleton; Antony Payton; Lynne Hampson; Ian Hampson; Janine Lamb

We evaluated the mean NGSRe-norm for each family. In DM, Nairoviridae (n=1), Poxviridae (n=8), Secoviridae (natural host: plants) (n=1), Caliciviridae (n=4) and Adenoviridae (n=1) were the top 5 ranked families (Table S3) . Poxviridae was the one family that ranked highly regarding both the NGSRe-norm (Table S3 ) and the family richness (n=8, 95%CI: 2.98-3.61) ( Table S2 ). All 8 pox viruses had high NSGRe-norm and were amongst the dominant DM viral species ( Figure 4F ). Specifically, Variola virus had the highest NSGRe-norm amongst all identified viral species ( Figure 4F ). In the HC P20, Polyomaviridae (n=1), Iflaviridae (n=1), Podoviridae (n=1), Tymoviridae (n=2), and Myoviridae (n=3) were the viral families with the highest mean NGSRe-norm. Podoviridae and Myoviridae are prokaryotic viruses infecting bacteria, Tymoviridae infect plants and Iflaviridae infect insects (ViralZone root-ExPASy) (Hulo et al., 2011) . We also used Z transformation of NGSRe-norm to define the precise location and rank of each viral family within the DM and HC distribution ( Figure 4D & 4E). In DM, multiple viral families (Nairoviridae, Poxviridae, Secoviridae, Alloherpesviridae, Caliciviridae and Adenoviridae) with high NGSRe-norm Z score occurred with at least one standard deviation above the group mean. In HC, only Polyomaviridae (n=1) occurred with high NGSRe-norm Z score. In Figure S4 we provide a cladogram of DM-specific viral species.