Selected article for: "care point and poc testing"

Author: Jennifer N Rauch; Eric Valois; Sabrina C Solley; Friederike Braig; Ryan S Lach; Naomi J Baxter; Kenneth S Kosik; Carolina Arias; Diego Acosta-Alvear; Maxwell Z Wilson
Title: A Scalable, Easy-to-Deploy, Protocol for Cas13-Based Detection of SARS-CoV-2 Genetic Material
  • Document date: 2020_4_21
  • ID: hy1xrw6q_13
    Snippet: The unparalleled spread of SARS-CoV-2 demands orthogonal solutions to expand testing capacities. Here, we demonstrate an optimized Cas13-based protocol that uses accessible reagents and affordable equipment, while maintaining sensitivity that is comparable to established testing methods. Considering the need for a rugged, equitable, and scalable test, we couple spartan, Bluetooth-enabled thermocyclers that can be plugged into a battery and run us.....
    Document: The unparalleled spread of SARS-CoV-2 demands orthogonal solutions to expand testing capacities. Here, we demonstrate an optimized Cas13-based protocol that uses accessible reagents and affordable equipment, while maintaining sensitivity that is comparable to established testing methods. Considering the need for a rugged, equitable, and scalable test, we couple spartan, Bluetooth-enabled thermocyclers that can be plugged into a battery and run using mobile device applications, with simple plastic-filter-based, LED visualizers. Results can be simply documented with a smartphone camera and uploaded to the cloud, enabling distributed point-of-care (POC) testing. Operational costs could be further lowered by multiplexing the amplification of viral targets.

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