Author: Ioanna Smyrlaki; Martin Ekman; Martin Vondracek; Natali Papanicoloau; Antonio Lentini; Johan Aarum; Shaman Muradrasoli; Jan Albert; Björn Högberg; Björn Reinius
Title: Massive and rapid COVID-19 testing is feasible by extraction-free SARS-CoV-2 RT-qPCR Document date: 2020_4_17
ID: jeufhpkq_38
Snippet: The copyright holder for this preprint (which was not peer-reviewed) is (e) Barplots of CT from SARS-CoV-2 RT-qPCR using the N1 primer-prove set performed directly on lysed donor samples a�er storage and freeze thaw from self-sampling (saliva or nasal + throat swab suspensions taken with co�on �pped wooden s�cks) without purifica�on. Percent Triton X-100 indicates the percentage detergent in the sample (half concentra�on in the RT-qPC.....
Document: The copyright holder for this preprint (which was not peer-reviewed) is (e) Barplots of CT from SARS-CoV-2 RT-qPCR using the N1 primer-prove set performed directly on lysed donor samples a�er storage and freeze thaw from self-sampling (saliva or nasal + throat swab suspensions taken with co�on �pped wooden s�cks) without purifica�on. Percent Triton X-100 indicates the percentage detergent in the sample (half concentra�on in the RT-qPCR reac�on). Crosses indicate CT values from diagnos�cs performed on extracted RNA from fresh aliquots.
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