Messenger RNAs transcribed from yeast linear cytoplasmic plasmids possess unconventional 5’ and 3’ UTRs and suggest a novel mechanism of translation

Václav Vopálenský; Michal Sýkora; Tomáš Mašek; Martin Pospíšek

Please follow the Supplemental experimental procedures for the rest of the methods including Plasmid construction, Assay of enzyme-GMP complex formation, Removal of mitochondrial DNA by ethidium bromide treatment, Transformation of yeast cells, Killer production in modified CWO4 yeast strains, Assay of killer toxin activity, Statistical analysis, Expression and purification of mRNA decapping enzymes, In vitro processing of mRNAs by decapping enzymes, In vitro synthesis of capped mRNA using the vaccinia virus mRNA capping enzyme, Deletion of PBP1, PAB1 and LSM1 genes and the full list of primers, strains and plasmids used throughout this study.