Author: Václav Vopálenský; Michal Sýkora; Tomáš Mašek; Martin Pospíšek
Title: Messenger RNAs transcribed from yeast linear cytoplasmic plasmids possess unconventional 5’ and 3’ UTRs and suggest a novel mechanism of translation Document date: 2018_5_17
ID: foskvkwn_38
Snippet: We developed a unique fusion PCR strategy, followed with homologous recombination in vivo; this approach enabled us to precisely modify the K2ORF3 MTase coding region directly in the cytoplasmically localized pGKL plasmids (for details of the method, see The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/325316 doi: bioRxiv preprint 2D). Four hundred generations of the mutated plasmid being favor.....
Document: We developed a unique fusion PCR strategy, followed with homologous recombination in vivo; this approach enabled us to precisely modify the K2ORF3 MTase coding region directly in the cytoplasmically localized pGKL plasmids (for details of the method, see The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/325316 doi: bioRxiv preprint 2D). Four hundred generations of the mutated plasmid being favored by selective pressure and cannot outcompete the wild-type plasmid is roughly the limit of the indispensability of any analyzed pGKL ORF in this kind of experiment (68).
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