Selected article for: "pool sample and positive pool sample"
Author: Kuiama Lewandowski; Yifei Xu; Steven T. Pullan; Sheila F. Lumley; Dona Foster; Nicholas Sanderson; Alison Vaughan; Marcus Morgan; Nicole Bright; James Kavanagh; Richard Vipond; Miles Carroll; Anthony C. Marriott; Karen E Gooch; Monique Andersson; Katie Jeffery; Timothy EA Peto; Derrick W. Crook; A Sarah Walker; Philippa C. Matthews
Title: Metagenomic Nanopore sequencing of influenza virus direct from clinical respiratory samples
  • Document date: 2019_6_19
    • ID: 75j8jydo_7
    Snippet: Starting with an influenza A positive sample pool (10 6 genome copies/ml), we made three 117 volumetric dilution series using three independent influenza-negative pools (Fig 1) . The total 118 quantity of cDNA after preparation for sequencing was consistently higher in all samples using 119 negative pool-3 as diluent (Fig 2A) , indicating the presence of a higher concentration of non-120 viral RNA within pool-3. This is likely due to host cell ly.....
    Document: Starting with an influenza A positive sample pool (10 6 genome copies/ml), we made three 117 volumetric dilution series using three independent influenza-negative pools (Fig 1) . The total 118 quantity of cDNA after preparation for sequencing was consistently higher in all samples using 119 negative pool-3 as diluent (Fig 2A) , indicating the presence of a higher concentration of non-120 viral RNA within pool-3. This is likely due to host cell lysis or higher bacterial presence, and 121 demonstrates the variable nature of throat swab samples. 122

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