Selected article for: "cdna complementary dna generate and complementary dna"
Author: C Langelier; MS Zinter; K Kalantar; GA Yanik; S Christenson; B Odonovan; C White; M Wilson; A Sapru; CC Dvorak; S Miller; CY Chiu; JL DeRisi
Title: Metagenomic Sequencing Detects Respiratory Pathogens in Hematopoietic Cellular Transplant Patients
  • Document date: 2017_1_24
    • ID: km0b1z64_9
    Snippet: Total nucleic acid was extracted from 250µl of patient BAL fluid using bead-based lysis and the Zymo Viral DNA/RNA Kit (Zymo Research). RNA and DNA were isolated separately using DNAse or RNAse, respectively, and the former was reverse transcribed to generate complementary DNA (cDNA). DNA and cDNA then underwent adapter addition and barcoding using the Nextera system (Illumina). Depletion of abundant sequences by hybridization (DASH) was employe.....
    Document: Total nucleic acid was extracted from 250µl of patient BAL fluid using bead-based lysis and the Zymo Viral DNA/RNA Kit (Zymo Research). RNA and DNA were isolated separately using DNAse or RNAse, respectively, and the former was reverse transcribed to generate complementary DNA (cDNA). DNA and cDNA then underwent adapter addition and barcoding using the Nextera system (Illumina). Depletion of abundant sequences by hybridization (DASH) was employed to selectively deplete human mitochondrial cDNA, thus enriching for microbial transcripts (20) . The final RNAseq and DNA sequencing (DNAseq) libraries underwent 135 nucleotide paired-end Illumina sequencing. Methodology is described further in the online data supplement.

    Search related documents:
    Co phrase search for related documents
    • cdna complementary dna and complementary dna: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
    • cdna complementary dna and DASH hybridization: 1
    • cdna complementary dna and human mitochondrial: 1
    • cdna complementary dna generate and complementary dna: 1, 2
    • cdna dna and complementary dna: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
    • cdna dna and DASH hybridization: 1
    • cdna dna and human mitochondrial: 1
    • complementary dna and DASH hybridization: 1
    • complementary dna and human mitochondrial: 1
    • DASH hybridization and human mitochondrial: 1