Author: Zhen Zhao; Haodong Cui; Wenxing Song; Xiaoling Ru; Wenhua Zhou; Xuefeng Yu
Title: A simple magnetic nanoparticles-based viral RNA extraction method for efficient detection of SARS-CoV-2 Document date: 2020_2_27
ID: nbu6bsb3_41
Snippet: Firstly, pcMNPs-based method combines the virus lysis and RNA binding steps into one, and the pcMNPs-RNA complexes can be directly introduced into subsequent RT-PCR reactions (Direct RT-PCR), which gives a dramatically simplified RNA extraction protocol. Secondly, pcMNPs have excellent viral RNA binding performances, which results in 10-copy sensitivity and the high linearity over 5 logs of gradient in SARS-CoV-2 viral RNA detection using RT-PCR......
Document: Firstly, pcMNPs-based method combines the virus lysis and RNA binding steps into one, and the pcMNPs-RNA complexes can be directly introduced into subsequent RT-PCR reactions (Direct RT-PCR), which gives a dramatically simplified RNA extraction protocol. Secondly, pcMNPs have excellent viral RNA binding performances, which results in 10-copy sensitivity and the high linearity over 5 logs of gradient in SARS-CoV-2 viral RNA detection using RT-PCR. Thirdly, this method can be easily adopted in fully automated nucleic acid extraction systems without laborious optimization.
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