Low basal expression and slow induction of IFITM3 puts immune cells at risk of influenza A infection

Dannielle Wellington; Zixi Yin; Liwei Zhang; Jessica Forbester; Kerry Kite; Henry Laurenson-Schafer; Shokouh Makvandi-Nejad; Boquan Jin; Emma Bowes; Krishnageetha Manoharan; David Maldonado-Perez; Clare Verill; Ian Humphreys; Tao Dong

Taken together, the data presented here shows that the IFITM3 582 expression pattern is influenced by cell type, location, differentiation state and 583 naivety with high variability possible. However, what is clear is that basal 584 IFITM3 expression is higher on myeloid than lymphoid cells and induction 585 mostly occurs in these cells following IFN stimulation, although this induction 586 can take up to 24 hours for marked increase in expression. The data here will 587 provide important information for the study of IFITM3 viral restriction in several 588 infection settings as well as aiding in research of the role of IFITM3 in cancer 589 and cytokine production. The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2019.12.20.885590 doi: bioRxiv preprint myeloid progenitor (CMP) / granulocyte-monocyte progenitors (GMP) and B 710 cell + NK cell progenitor (B/NK Pro). Data is expressed ±SEM. Adult blood 711 donors n=3, lung tissue samples n=5, cord blood samples n=3, cell line data 712