Author: Václav Vopálenský; Michal Sýkora; Tomáš Mašek; Martin Pospíšek
Title: Messenger RNAs transcribed from yeast linear cytoplasmic plasmids possess unconventional 5’ and 3’ UTRs and suggest a novel mechanism of translation Document date: 2018_5_17
ID: foskvkwn_65
Snippet: The lack of eIF4E binding to pGKL mRNAs in vitro prompted us to test whether these mRNAs require a functional cap-dependent pathway for their translation initiation. The reliable determination of whether a specific mRNA is translated cap-independently in vivo is not easy (100). We employed yeast strains bearing conditional mutations in the CDC33 gene, which codes for eIF4E. S. cerevisiae CWO4 strains carrying either a cdc33-1 or cdc33-42 allele a.....
Document: The lack of eIF4E binding to pGKL mRNAs in vitro prompted us to test whether these mRNAs require a functional cap-dependent pathway for their translation initiation. The reliable determination of whether a specific mRNA is translated cap-independently in vivo is not easy (100). We employed yeast strains bearing conditional mutations in the CDC33 gene, which codes for eIF4E. S. cerevisiae CWO4 strains carrying either a cdc33-1 or cdc33-42 allele as a sole source of the eIF4E translation initiation factor (kindly obtained from Prof.
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