Author: Václav Vopálenský; Michal Sýkora; Tomáš Mašek; Martin Pospíšek
Title: Messenger RNAs transcribed from yeast linear cytoplasmic plasmids possess unconventional 5’ and 3’ UTRs and suggest a novel mechanism of translation Document date: 2018_5_17
ID: foskvkwn_68_1
Snippet: in from the pYX212::M1 plasmid declines at the end of exponential growth (108), and this phenomenon is even more profound in the version of the TPI promoter used in the pYX212 plasmid (unpublished data). The growth of the CWO4 CDC33, Ï 0 [pYX212::M1] strain is slightly faster at 37°C than at 24°C, causing the cells to reach early stationary phase in 8 hours (instead of 12 at 24°C); this difference explains why K1 toxin production in the CDC33.....
Document: in from the pYX212::M1 plasmid declines at the end of exponential growth (108), and this phenomenon is even more profound in the version of the TPI promoter used in the pYX212 plasmid (unpublished data). The growth of the CWO4 CDC33, Ï 0 [pYX212::M1] strain is slightly faster at 37°C than at 24°C, causing the cells to reach early stationary phase in 8 hours (instead of 12 at 24°C); this difference explains why K1 toxin production in the CDC33 wild-type strain ceases in samples grown for 12 hours at 37°C (Fig. 6B ).
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