Kinetic Analysis of Bacteriophage Sf6 Binding to Outer Membrane Protein A Using Whole Virions

Natalia B. Hubbs; Mareena M. Whisby-Pitts; Jonathan L. McMurry

Surface plasmon resonance (SPR), another optical biosensing technique, has also been used to study virus:host interactions. For example, Bonaparte et al. showed that the equilibrium dissociation constant for Hendra virus attachment glycoprotein to its receptor, human ephrin-B2 is 1 nM [34] . Another SPR study showed that purified receptor binding proteins of human coronavirus, Middle East respiratory syndrome (MERS-CoV), and a bat coronavirus HKU4 can bind to human CD26 with K D s of 18.4 nM and 35.7 μM, respectively [35] . Recently, Marti et al. showed via SPR that the binding affinity of the long tail fiber of bacteriophage S16, the phage tail protein that mediates interaction with the host, and its host Salmonella enterica ssp. enterica is ~ 5 nM [12] .