Mammalian orthoreovirus infection is enhanced in cells pre-treated with sodium arsenite

Michael M. Lutz; Megan P. Worth; Meleana M. Hinchman; John S. L. Parker; Emily D. Ledgerwood

reovirus strains Type 1 Lang (T1L), Type 2 Jones (T2J), Type 3 Abney (T3A), and Type 306 3 Dearing (T3D) differ in their capacity to induce host translational shutoff and to induce 307 eIF2α phosphorylation [15, 26] . To determine if the benefit of pre-treatment with sodium 308 arsenite was reovirus strain specific, we infected CV-1 cells with T3D, T1L or T3A and 309 assessed the percentage of infected cells at 18 h p.i. compared to untreated cells. As 310 observed following infection with the T3D strain, pre-treatment with sodium arsenite 311 resulted in nearly a 2-fold increase in the percentage of T1L-and T3A-infected cells 312 ( Figure 3B ). Sodium arsenite pre-treatment in L929 cells infected with T3D, T1L or T3A 313 yielded similar results, however sodium arsenite pre-treatment had no effect on any strain 314 tested in HeLa cells (Supplemental Figure 1) . These data suggest that reovirus strains 315 T3D, T3A and T1L benefit from pre-treatment with sodium arsenite prior to infection in 316 some cell types. 317 318 Heat shock prior to infection enhances viral protein expression in T3D-infected L-319