Microbial and autoantibody immunogenic repertoires in TIF1? autoantibody positive dermatomyositis

Spyridon Megremis; Thomas Walker; Xiaotong He; James O'Sullivan; William E.R. Ollier; Hector Chinoy; Neil Pendleton; Antony Payton; Lynne Hampson; Ian Hampson; Janine Lamb

. CC-BY-ND 4.0 International license author/funder. It is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.03.25.007534 doi: bioRxiv preprint The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.03.25.007534 doi: bioRxiv preprint factors in the meta-analysis we defined NGSRe-norm. (E) Optical density measurements during the biopanning process. The optical density of the FliTrx E. Coli cells was measured after each round of panning. No differences were observed between the DM and HC cross-panning pairs. Welch's t test p value 0.861 for P20 comparison and 0.954 for P10. (F) DNA chromatograph of amplified variance region. Illustrative variance region following Sanger sequencing. Portion of variance region is ¼ the intensities of flanking consensus along the 36bp variance region as a function of each measured base ("N") comprising equal quantities of A, C, T, and G nucleotides. The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.03.25.007534 doi: bioRxiv preprint Supplementary figure 6: The dermatomyositis-specific autoantibody module. Human proteome autoantibody-targets that were identified in the DM P20 were used to screen for GO enrichment (biological processes). The distribution of the identified proteins in the GO processes that were successfully represented in the DM-enriched autoantibody proteome along with the GO-coverage (heatmap) in DM and HC can be seen in (A). Graph representation of the DM proteome module (B):