Selected article for: "gene expression and wild type"

Author: Gloria P. Larson; Vy Tran; Shuiqìng Yú; Yíngyún Caì; Christina A. Higgins; Danielle M. Smith; Steven F. Baker; Sheli R. Radoshitzky; Jens H. Kuhn; Andrew Mehle
Title: EPS8 facilitates uncoating of influenza A virus
  • Document date: 2019_3_28
  • ID: muq5rkaa_42
    Snippet: Acid bypass with WSN PASTN was performed as described (Matlin et al., 1981; Mondal et al., 2017) . Wild type and EPS8-edited A549 cells were inoculated at an MOI of 0.1 with virus diluted in VGM with 0.25 μg/ml TPCK-trypsin for 1 hour at 4°C. The inoculum was removed and cells were washed with cold Dulbecco's phosphate-buffered saline (DPBS). Inoculated cells were then either treated with 20 mM HEPES, pH 7.4 in 154 mM NaCl or 50 mM citrate, pH .....
    Document: Acid bypass with WSN PASTN was performed as described (Matlin et al., 1981; Mondal et al., 2017) . Wild type and EPS8-edited A549 cells were inoculated at an MOI of 0.1 with virus diluted in VGM with 0.25 μg/ml TPCK-trypsin for 1 hour at 4°C. The inoculum was removed and cells were washed with cold Dulbecco's phosphate-buffered saline (DPBS). Inoculated cells were then either treated with 20 mM HEPES, pH 7.4 in 154 mM NaCl or 50 mM citrate, pH 5.0 in 154 mM NaCl for 45 seconds at 37°C. The inoculum and treatment buffer were removed and cells were washed with room temperature DPBS. Pre-warmed DMEM supplemented with 10% FBS was added to the cells, infection progressed at 37°C for 8 hours, and viral gene expression was measured by a Nano-Glo assay.

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