Author: Hulda R. Jonsdottir; Sabrina Marti; Dirk Geerts; Regulo Rodriguez; Volker Thiel; Ronald Dijkman
Title: Establishment of primary transgenic human airway epithelial cell cultures to study respiratory virus – host interactions Document date: 2019_7_8
ID: 33yyg2z5_1
Snippet: However, in order to fully utilize the potential of this culture system it must be made amenable to 66 genetic modification. Transgenesis would enable the study of viral and/or host factors important for 67 respiratory virus infections and allow for the elucidation of specific mechanism involved in virus-host 68 interactions by targeted gene knockdown or overexpression. However, using primary cells for genetic 69 modification is challenging since.....
Document: However, in order to fully utilize the potential of this culture system it must be made amenable to 66 genetic modification. Transgenesis would enable the study of viral and/or host factors important for 67 respiratory virus infections and allow for the elucidation of specific mechanism involved in virus-host 68 interactions by targeted gene knockdown or overexpression. However, using primary cells for genetic 69 modification is challenging since they have a finite life span in cell culture. Primary human bronchial 70 cells can only be limitedly passaged after isolation if differentiation capabilities are to be maintained 71 [16] . The incorporation of the Rho-associated protein kinase (ROCK) inhibitor Y-27632 has been 72 shown to increase the number of passages primary cells can undergo in vitro without gross influence 73 on cell differentiation capacity [17, 18] . Theoretically, this would enable the generation of genetically 74 modified well-differentiated primary human airway epithelium in vitro.
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