Author: Václav Vopálenský; Michal Sýkora; Tomáš Mašek; Martin Pospíšek
Title: Messenger RNAs transcribed from yeast linear cytoplasmic plasmids possess unconventional 5’ and 3’ UTRs and suggest a novel mechanism of translation Document date: 2018_5_17
ID: foskvkwn_15
Snippet: Two microliters of reverse transcriptase reaction (obtained from eIF4E-mRNA in vitro binding assay) were subjected to PCR amplification as follows (5 min at 94°C; then 35 cycles of 30 sec at 94°C; 30 sec at 50°C; 45 sec at 72°C; and finally, 4 min at 72°C) using Taq DNA polymerase (Roche) and HGT1 and K2ORF5 gene-specific primers (listed in Table S2 )......
Document: Two microliters of reverse transcriptase reaction (obtained from eIF4E-mRNA in vitro binding assay) were subjected to PCR amplification as follows (5 min at 94°C; then 35 cycles of 30 sec at 94°C; 30 sec at 50°C; 45 sec at 72°C; and finally, 4 min at 72°C) using Taq DNA polymerase (Roche) and HGT1 and K2ORF5 gene-specific primers (listed in Table S2 ).
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