Selected article for: "cc NC International license and large scale"
Author: Jonathan L Schmid-Burgk; David Li; David Feldman; Mikolaj Slabicki; Jacob Borrajo; Jonathan Strecker; Brian Cleary; Aviv Regev; Feng Zhang
Title: LAMP-Seq: Population-Scale COVID-19 Diagnostics Using a Compressed Barcode Space
  • Document date: 2020_4_8
    • ID: 68ps3uit_7
    Snippet: We propose the following approach for population-scale testing for SARS-CoV-2 infection: a barcoded RT-LAMP reaction is performed on an unpurified swab sample with primers specific for the SARS-CoV-2 genome, which is followed by large-scale pooling of samples, PCR amplification with additional barcoding, deep sequencing, and data analysis to identify positive individuals ( Fig. 1A ) (see below for detailed suggested protocol). Advantages of perfo.....
    Document: We propose the following approach for population-scale testing for SARS-CoV-2 infection: a barcoded RT-LAMP reaction is performed on an unpurified swab sample with primers specific for the SARS-CoV-2 genome, which is followed by large-scale pooling of samples, PCR amplification with additional barcoding, deep sequencing, and data analysis to identify positive individuals ( Fig. 1A ) (see below for detailed suggested protocol). Advantages of performing the RT-LAMP reaction at the site of sample collection include eliminating RNA extraction and . CC-BY-NC-ND 4.0 International license author/funder. It is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.04.06.025635 doi: bioRxiv preprint 5 concerns about RNA stability, providing sterilization before shipment, and allowing parallel logistics of large numbers of samples through remote sample pooling.

    Search related documents:
    Co phrase search for related documents
    • barcode RT LAMP reaction and population scale: 1
    • barcode RT LAMP reaction and population scale testing: 1
    • barcode RT LAMP reaction and RT LAMP reaction: 1
    • deep sequencing and large number: 1, 2, 3, 4, 5, 6, 7
    • deep sequencing and large scale: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10
    • deep sequencing and PCR amplification: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15
    • deep sequencing and population scale: 1
    • deep sequencing and population scale testing: 1
    • deep sequencing and RNA extraction: 1, 2
    • follow approach and large number: 1, 2
    • follow approach and large scale: 1
    • large number and PCR amplification: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10
    • large number and population scale: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13
    • large number and population scale testing: 1, 2, 3, 4, 5, 6, 7
    • large number and RNA extraction: 1, 2, 3
    • large number and RNA stability: 1
    • large scale and positive individual: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13
    • large scale and RNA extraction: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
    • large scale and RNA stability: 1, 2