Selected article for: "cell lysate and vero cell"
Author: Cheng, Man; Chan, Ceci W.L.; Cheung, Randy C.F.; Bikkavilli, Rama Kamesh; Zhao, Qi; Au, Shannon W.N.; Chan, Paul K.S.; Lee, Susanna S.T.; Cheng, Gregory; Ho, Walter K.K.; Cheung, Wing-Tai
Title: Cross-reactivity of antibody against SARS-coronavirus nucleocapsid protein with IL-11
  • Cord-id: tlnao8y3
  • Document date: 2005_12_23
    • ID: tlnao8y3
    Snippet: Infection of SARS-associated coronavirus (SARS-CoV) induced a strong anti-nucleocapsid (anti-N) antibody response. However, the pathophysiological significance of the anti-N antibodies in SARS pathogenesis is largely unknown. To profile the anti-N antibodies, a phage-displayed scFv library was prepared from mice immunized with heat-inactivated SARS-CoV-infected Vero E6 cell lysate. Specific anti-N scFvs were isolated by panning against a recombinant nucleocapsid protein and reactivity was confir
    Document: Infection of SARS-associated coronavirus (SARS-CoV) induced a strong anti-nucleocapsid (anti-N) antibody response. However, the pathophysiological significance of the anti-N antibodies in SARS pathogenesis is largely unknown. To profile the anti-N antibodies, a phage-displayed scFv library was prepared from mice immunized with heat-inactivated SARS-CoV-infected Vero E6 cell lysate. Specific anti-N scFvs were isolated by panning against a recombinant nucleocapsid protein and reactivity was confirmed with phage-ELISA. Sequence analysis indicated that two of the isolated anti-N scFv clones were identical and displayed a high homology with an scFv specific for interleukin 11 (IL-11), an anti-inflammatory cytokine derived from bone marrow stroma cells. In a neutralization assay, IL-11-induced STAT 3 phosphorylation in rat intestinal epithelial IEC-18 cells was completely suppressed by the anti-N scFv clone L9N01.

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