Author: Deepak Kumar; Nitin Sharma; Murali Aarthy; Sanjeev Singh; Rajanish Giri
Title: Mechanistic insights into Zika virus NS3 helicase inhibition by Epigallocatechin-3-gallate: Supplementary Files Document date: 2019_1_26
ID: k11iupe0_18
Snippet: The E. coli expressed recombinant NS3 helicase (53.6kDa) was purified by Ni-NTA affinity chromatography as shown in figure 5A . ATPase activity of NS3 helicase was determined by absorbance (630nm) based malachite green method which estimates the release of free phosphate......
Document: The E. coli expressed recombinant NS3 helicase (53.6kDa) was purified by Ni-NTA affinity chromatography as shown in figure 5A . ATPase activity of NS3 helicase was determined by absorbance (630nm) based malachite green method which estimates the release of free phosphate.
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