Microbial and autoantibody immunogenic repertoires in TIF1? autoantibody positive dermatomyositis

Spyridon Megremis; Thomas Walker; Xiaotong He; James O'Sullivan; William E.R. Ollier; Hector Chinoy; Neil Pendleton; Antony Payton; Lynne Hampson; Ian Hampson; Janine Lamb

. CC-BY-ND 4.0 International license author/funder. It is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.03.25.007534 doi: bioRxiv preprint Epitope signature set analysis: M5 Distinct epitope signature set analysis presented unique and common AA epitope sequence sets with associated NGS enrichment scores. Minimum thresholds of 10AA sequence length and the 10-σ-99 NGS noise filter provided sensitive annotation power while minimising annotation noise ( Figure S1D -S1F). Cleaned distinct epitope sequences were collated. Unique epitope pools were determined by symmetric difference of the HC and DM pools (i.e. specific epitope sequences uniquely found in HC or in DM patients). The intersect between HC and DM pools was probed and a minimum five-fold change threshold applied to partition relevant epitope collections to supplement the HC or DM pools.