Microbial and autoantibody immunogenic repertoires in TIF1? autoantibody positive dermatomyositis

Spyridon Megremis; Thomas Walker; Xiaotong He; James O'Sullivan; William E.R. Ollier; Hector Chinoy; Neil Pendleton; Antony Payton; Lynne Hampson; Ian Hampson; Janine Lamb

Continuous variables were log10 transformed and tested for normal distribution using the Shapiro-Wilk test. Pairwise quantitative differences between two groups were tested using two-tailed Mann Whitney (Wilcoxon rank sum test) for variables not following a normal distribution and unpaired t test for variables with a normal distribution. F test was used to test for equal variance. The Kruskal-Wallis nonparametric test was used to compare three or more groups; Dunn's multiple comparisons test was used to compare pairwise differences in the sum of ranks with the expected average difference (based on the number of groups and sample size per group). Z score transformation was used to specify the location of each observation within a distribution. Statistical analyses were performed with GraphPad Prism 8.0 (GraphPad Software, Inc., San Diego, CA)(GraphPad Software).