Author: Iyer, Anita S.; Jones, Forrest K.; Nodoushani, Ariana; Kelly, Meagan; Becker, Margaret; Slater, Damien; Mills, Rachel; Teng, Erica; Kamruzzaman, Mohammad; Garcia-Beltran, Wilfredo F.; Astudillo, Michael; Yang, Diane; Miller, Tyler E.; Oliver, Elizabeth; Fischinger, Stephanie; Atyeo, Caroline; Iafrate, A. John; Calderwood, Stephen B.; Lauer, Stephen A.; Yu, Jingyou; Li, Zhenfeng; Feldman, Jared; Hauser, Blake M.; Caradonna, Timothy M.; Branda, John A.; Turbett, Sarah E.; LaRocque, Regina C.; Mellon, Guillaume; Barouch, Dan H.; Schmidt, Aaron G.; Azman, Andrew S.; Alter, Galit; Ryan, Edward T; Harris, Jason B.; Charles, Richelle C.
Title: Dynamics and significance of the antibody response to SARS-CoV-2 infection Cord-id: y5j08qu7 Document date: 2020_7_20
ID: y5j08qu7
Snippet: BACKGROUND: Characterizing the humoral immune response to SARS-CoV-2 and developing accurate serologic assays are needed for diagnostic purposes and estimating population-level seroprevalence. METHODS: We measured the kinetics of early antibody responses to the receptor-binding domain (RBD) of the spike (S) protein of SARS-CoV-2 in a cohort of 259 symptomatic North American patients infected with SARS-CoV-2 (up to 75 days after symptom onset) compared to antibody levels in 1548 individuals whose
Document: BACKGROUND: Characterizing the humoral immune response to SARS-CoV-2 and developing accurate serologic assays are needed for diagnostic purposes and estimating population-level seroprevalence. METHODS: We measured the kinetics of early antibody responses to the receptor-binding domain (RBD) of the spike (S) protein of SARS-CoV-2 in a cohort of 259 symptomatic North American patients infected with SARS-CoV-2 (up to 75 days after symptom onset) compared to antibody levels in 1548 individuals whose blood samples were obtained prior to the pandemic. RESULTS: Between 14–28 days from onset of symptoms, IgG, IgA, or IgM antibody responses to RBD were all accurate in identifying recently infected individuals, with 100% specificity and a sensitivity of 97%, 91%, and 81% respectively. Although the estimated median time to becoming seropositive was similar across isotypes, IgA and IgM antibodies against RBD were short-lived with most individuals estimated to become seronegative again by 51 and 47 days after symptom onset, respectively. IgG antibodies against RBD lasted longer and persisted through 75 days post-symptoms. IgG antibodies to SARS-CoV-2 RBD were highly correlated with neutralizing antibodies targeting the S protein. No cross-reactivity of the SARS-CoV-2 RBD-targeted antibodies was observed with several known circulating coronaviruses, HKU1, OC 229 E, OC43, and NL63. CONCLUSIONS: Among symptomatic SARS-CoV-2 cases, RBD-targeted antibodies can be indicative of previous and recent infection. IgG antibodies are correlated with neutralizing antibodies and are possibly a correlate of protective immunity.
Search related documents:
Co phrase search for related documents- accurate diagnosis and long term follow: 1
- accurate diagnosis and longitudinal cohort: 1
- accurate diagnosis and low prevalence: 1, 2
- accurate diagnosis facilitate and acute sars respiratory syndrome coronavirus: 1, 2, 3
- acute sars respiratory syndrome coronavirus and additional detail: 1
- acute sars respiratory syndrome coronavirus and long term follow: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44
- acute sars respiratory syndrome coronavirus and longitudinal cohort: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
- acute sars respiratory syndrome coronavirus and low prevalence: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72
- acute sars respiratory syndrome coronavirus and low prevalence area: 1, 2, 3, 4
- long term follow and low prevalence: 1
- longitudinal cohort and low prevalence: 1, 2, 3
Co phrase search for related documents, hyperlinks ordered by date