Selected article for: "flow cytometry and room temperature"
Author: Randall Toy; Pallab Pradhan; Vijayeetha Ramesh; Nelson C. Di Paolo; Blake Lash; Jiaying Liu; Emmeline L. Blanchard; Philip J. Santangelo; Dmitry M. Shayakhmetov; Krishnendu Roy
Title: Modification of primary amines to higher order amines reduces in vivo hematological and immunotoxicity of cationic nanocarriers through TLR4 and complement pathways
  • Document date: 2019_5_24
    • ID: cbit5xci_16
    Snippet: The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. . https://doi.org/10.1101/647305 doi: bioRxiv preprint RAW 264.7 macrophages were seeded in 96 well plates at a density of 30,000 cells/well. Cells were treated with 1.8 µg of nanoparticles for 30 minutes. At 30 minutes, cells were washed three times with PBS, fixed with 1% paraformaldehyde in PBS for 10 minutes at room temperature, and permeabilized with .....
    Document: The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. . https://doi.org/10.1101/647305 doi: bioRxiv preprint RAW 264.7 macrophages were seeded in 96 well plates at a density of 30,000 cells/well. Cells were treated with 1.8 µg of nanoparticles for 30 minutes. At 30 minutes, cells were washed three times with PBS, fixed with 1% paraformaldehyde in PBS for 10 minutes at room temperature, and permeabilized with 0 .2% TritonX-100 for 5 minutes at room temperature. PLA was then performed between TLR4 (Novus NB100-56581, 1:50 dilution) and TIRAP (Novus NB300-990, 1:100 dilution) and analyzed via flow cytometry using a DuoLink PLA flow cytometry kit (Sigma Aldrich, St. Louis, MO). Samples were analyzed using a BD Accuri C6

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