Author: Xiankun Zeng; Jun Liu; April Babka; Brian Kearney; Sheli Radoshitzky; Jens Kuhn
Title: Molecular Detection of SARS-CoV-2 in Formalin Fixed Paraffin Embedded Specimens Document date: 2020_4_22
ID: nzwe7hpg_12
Snippet: Here, we describe the evaluation of a rabbit polyclonal anti-SARS-CoV S antibody and a mouse 74 monoclonal anti-SARS-CoV NP antibody that are commercially available and, in IHC and IFA, 75 recognized respective SARS-CoV-2 proteins in FFPE specimens. We also identify two 76 commercially available ISH assays that can be used to efficiently detect SARS-CoV-2 RNA in 77 such specimens and develop a dual staining assay using IHC and ISH to detect SARS-.....
Document: Here, we describe the evaluation of a rabbit polyclonal anti-SARS-CoV S antibody and a mouse 74 monoclonal anti-SARS-CoV NP antibody that are commercially available and, in IHC and IFA, 75 recognized respective SARS-CoV-2 proteins in FFPE specimens. We also identify two 76 commercially available ISH assays that can be used to efficiently detect SARS-CoV-2 RNA in 77 such specimens and develop a dual staining assay using IHC and ISH to detect SARS-CoV-2 S 78 and RNA in the same FFPE section. 79 author/funder. All rights reserved. No reuse allowed without permission.
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