Author: Chunxi Zeng; Xucheng Hou; Jingyue Yan; Chengxiang Zhang; Wenqing Li; Weiyu Zhao; Shi Du; Yizhou Dong
Title: Leveraging mRNAs sequences to express SARS-CoV-2 antigens in vivo Document date: 2020_4_5
ID: aju2nr9x_40
Snippet: The mRNAs encoding SARS-CoV-2 antigens, RBD and S, were formulated by TT3 nanoparticles to 0.03 mg/mL and 0.06 mg/mL, respectively. The formulated nanoparticles were dialyzed in 1×PBS. The formulation was injected intramuscularly 15µg RBDencoding mRNA or 30µg S-encoding mRNA. Mice were euthanized six hours after injection. The muscle tissue at the injection sites was harvested and incubated in 4% paraformaldehyde overnight, followed by 30% suc.....
Document: The mRNAs encoding SARS-CoV-2 antigens, RBD and S, were formulated by TT3 nanoparticles to 0.03 mg/mL and 0.06 mg/mL, respectively. The formulated nanoparticles were dialyzed in 1×PBS. The formulation was injected intramuscularly 15µg RBDencoding mRNA or 30µg S-encoding mRNA. Mice were euthanized six hours after injection. The muscle tissue at the injection sites was harvested and incubated in 4% paraformaldehyde overnight, followed by 30% sucrose incubation overnight. The tissue was sectioned using Cryotome E Cryostat (Thermo-Shandon) and fixed using acetone. The subsequent staining procedure was the same as that for staining 293T cells. Images were taken on a Nikon A1R Live Cell Confocal microscope with NIS-Elements BR imaging software (version 4.20).
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