More than efficacy revealed by single-cell analysis of antiviral therapeutics

Wu Liu; Mehmet U. Caglar; Zhangming Mao; Andrew Woodman; Jamie J. Arnold; Claus O. Wilke; Craig E. Cameron

The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. . https://doi.org/10.1101/606715 doi: bioRxiv preprint infections established by 22 ± 4% with an IC50 value of 30 ± 8 nM (Fig. 3a) . An earlier study 181 did not observe an impact of GA prior to virus assembly; however, it is possible that a reduction 182 of the magnitude shown here would be concealed by experimental error (Geller et al., 2007) . 183 Because infection is monitored by eGFP, which requires virus entry, genome replication, and 184 genome translation, interference with any of these steps would lead to a reduction in the number 185 of eGFP-positive cells. It is clear, however, that translation and folding of eGFP are not altered in 186 the presence of the highest concentration of GA used in this experiment (Fig. S5) . 187