Selected article for: "Clean RNA kit and decapping enzyme"
Author: Václav Vopálenský; Michal Sýkora; Tomáš Mašek; Martin Pospíšek
Title: Messenger RNAs transcribed from yeast linear cytoplasmic plasmids possess unconventional 5’ and 3’ UTRs and suggest a novel mechanism of translation
  • Document date: 2018_5_17
    • ID: foskvkwn_130
    Snippet: In a 100 µl reaction, 500 ng of total RNA purified from K. lactis IFO1267 or 4.1 pmol of in vitro transcribed and capped RNA were incubated with 0.25 µg of recombinant hDcp2 or 0.25 µg of recombinant Rai1 enzyme in 1x decapping buffer (10 mM Tris-HCl, pH 7.5; 100 mM potassium acetate; 2 mM magnesium acetate; 0.5 mM MnCl 2 ; 2 mM dithiothreitol and 0.1 mM spermine) at 37°C for 30 min. After incubation, RNA was purified using an RNA Clean & Con.....
    Document: In a 100 µl reaction, 500 ng of total RNA purified from K. lactis IFO1267 or 4.1 pmol of in vitro transcribed and capped RNA were incubated with 0.25 µg of recombinant hDcp2 or 0.25 µg of recombinant Rai1 enzyme in 1x decapping buffer (10 mM Tris-HCl, pH 7.5; 100 mM potassium acetate; 2 mM magnesium acetate; 0.5 mM MnCl 2 ; 2 mM dithiothreitol and 0.1 mM spermine) at 37°C for 30 min. After incubation, RNA was purified using an RNA Clean & Concentrator-5 kit (Zymo Research) and analyzed by 5' RACE.

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