Author: Václav Vopálenský; Michal Sýkora; Tomáš Mašek; Martin Pospíšek
Title: Messenger RNAs transcribed from yeast linear cytoplasmic plasmids possess unconventional 5’ and 3’ UTRs and suggest a novel mechanism of translation Document date: 2018_5_17
ID: foskvkwn_39
Snippet: We purified pRKL2 plasmids from both types of yeast clones (Fig. 2, lanes 2 and 3) and sequenced the MTase coding regions of their K2ORF3 genes. Surprisingly, yeast clones containing pRKL2 and pGKL1 only carried mutations that reversed the K2Orf3p SAMbinding site to its wild-type sequence or contained incomplete mutational cassettes that brought the same result, leaving the SAM-binding site unmodified. We called these former plasmids pRKL2-1 (Fig.....
Document: We purified pRKL2 plasmids from both types of yeast clones (Fig. 2, lanes 2 and 3) and sequenced the MTase coding regions of their K2ORF3 genes. Surprisingly, yeast clones containing pRKL2 and pGKL1 only carried mutations that reversed the K2Orf3p SAMbinding site to its wild-type sequence or contained incomplete mutational cassettes that brought the same result, leaving the SAM-binding site unmodified. We called these former plasmids pRKL2-1 (Fig. 2D, lane 3) . The pRKL2-2 plasmids, which were prevalent, retained the mutations in the MTase active-site-coding region and required wild-type pGKL2 to be stably maintained in the cell.
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