Messenger RNAs transcribed from yeast linear cytoplasmic plasmids possess unconventional 5’ and 3’ UTRs and suggest a novel mechanism of translation

Václav Vopálenský; Michal Sýkora; Tomáš Mašek; Martin Pospíšek

In both cases, the results are shown for S. cerevisiae CWO4ρ 0 strains bearing either the wildtype CDC33 (eIF4E) gene (CDC33wt) or temperature-sensitive mutations in the CDC33 (eIF4E) gene (cdc33-1 and cdc33-42 alleles) grown under both permissive (24°C) and nonpermissive (37°C) conditions. Aliquots were taken at 0, 3, 6 and 12 hours, and the culture medium was filter-sterilized, diluted 10x, and assayed for the presence of the killer toxin . CC-BY-NC-ND 4.0 International license author/funder. It is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/325316 doi: bioRxiv preprint activity by an agar well diffusion test using a lawn of S. cerevisiae S6/1 sensitive strain cells. Growth curves of all strains at permissive (blue) and non-permissive (purple) temperatures are shown below each of the corresponding sets of killer toxin experiments.