Author: Aran Singanayagam; Joseph Footitt; Benjamin T Kasdorf; Matthias Marczynski; Michael T Cross; Lydia J Finney; Maria-Belen Trujillo Torralbo; Maria Calderazzo; Jie Zhu; Julia Aniscenko; Thomas B Clarke; Philip L Molyneaux; Nathan W Bartlett; Miriam F Moffatt; William O Cookson; Jadwiga Wedzicha; Christopher M Evans; Oliver Lieleg; Patrick Mallia; Sebastian L Johnston
Title: MUC5AC drives COPD exacerbation severity through amplification of virus-induced airway inflammation Document date: 2019_7_22
ID: gg2ctmn7_67
Snippet: The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/706804 doi: bioRxiv preprint MUC5AC and MUC5B proteins in previously stored human sputum plugs or human/mouse BAL were measured after adhesion to a 96 well plate by allowing samples to evaporate at 37 o C overnight, using in-house assays as previously described 47 . For measurement of MUC5AC, the detection antibody used was biotinylated anti-MUC5.....
Document: The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/706804 doi: bioRxiv preprint MUC5AC and MUC5B proteins in previously stored human sputum plugs or human/mouse BAL were measured after adhesion to a 96 well plate by allowing samples to evaporate at 37 o C overnight, using in-house assays as previously described 47 . For measurement of MUC5AC, the detection antibody used was biotinylated anti-MUC5AC (ThermoScientific, UK) at 400 ng/mL. For the MUC5B assay, detection antibody was mouse anti-MUC5B clone EH-Muc5ba, as previously described 48 . Bound anti-MUC5B antibody was detected with peroxidase-conjugated goat anti-mouse IgG (Sigma-Aldrich, UK). Mucins were quantified against the following standards: purified porcine MUC5AC, as previously described 45 and recombinant MUC5B protein (Caltag Medsystems, UK).
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