Author: Kenneth Lyon; Luis U. Aguilera; Tatsuya Morisaki; Brian Munsky; Timothy J. Stasevich
Title: Live-cell single RNA imaging reveals bursts of translational frameshifting Document date: 2018_11_24
ID: 4fm1skgh_70
Snippet: Movie S1. Dynamics of cell presented in Figure 1B Max projection of a 13 z-stack movie showing an example U-2 OS cell expressing the +FSS multi-frame tag and bead-loaded with probes: MCP-Halo JF646 (Ch1 red, RNA), FLAG Fab Cy3 (Ch2 green, 0 ORF), and scFv-sfGFP (Ch3 blue, -1 ORF). Images were acquired every 4 seconds for a total of 76 seconds. Circled regions of interest correspond to species identified in Figure 1B and C. Scale bar, 10 µm......
Document: Movie S1. Dynamics of cell presented in Figure 1B Max projection of a 13 z-stack movie showing an example U-2 OS cell expressing the +FSS multi-frame tag and bead-loaded with probes: MCP-Halo JF646 (Ch1 red, RNA), FLAG Fab Cy3 (Ch2 green, 0 ORF), and scFv-sfGFP (Ch3 blue, -1 ORF). Images were acquired every 4 seconds for a total of 76 seconds. Circled regions of interest correspond to species identified in Figure 1B and C. Scale bar, 10 µm.
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